A: Disarmed Ti plasmid can be utilised as a s | vedclass

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Question

(A) The $Ti$ plasmid (Tumor-inducing plasmid) is naturally found in $Agrobacterium$ $tumefaciens$. By removing the tumor-causing genes,it becomes a 'd

Vedclass · Accepted Answer

Assertion and Reason both are correct and Reason is the correct explanation of Assertion.

Vedclass · Answer

(A) The $Ti$ plasmid (Tumor-inducing plasmid) is naturally found in $Agrobacterium$ $tumefaciens$. By removing the tumor-causing genes,it becomes a 'disarmed' $Ti$ plasmid. $A$ shuttle vector is defined as a vector that can replicate in two different host organisms (e.g.,both prokaryotes like $E. coli$ and eukaryotes like plants). Since the disarmed $Ti$ plasmid can be maintained in $E. coli$ (prokaryote) and also used to transform plant cells (eukaryote),it functions as a shuttle vector. Therefore,both the Assertion and the Reason are correct,and the Reason correctly explains why it is called a shuttle vector.

$A$: Disarmed $Ti$ plasmid can be utilised as a shuttle vector.
$R$: It can survive in both prokaryotic and eukaryotic organisms.

Similar Questions

Assertion : Plasmids are extrachromosomal $DNA$.
Reason : Plasmids are found in bacteria and are useful in genetic engineering.

Identify the plasmid among the following.

$A$ - Cloning vectors are useful to transfer desirable $DNA$ into host cells.
$R$ - Agrobacterium tumefaciens is a pathogen of several dicot plants.

Assertion : Restriction enzymes cut the strand of $DNA$ to produce sticky ends.
Reason : Stickiness of the ends facilitates the action of the enzyme $DNA$ polymerase.

What is true for plasmid?

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$A$: Disarmed $Ti$ plasmid can be utilised as a shuttle vector.$R$: It can survive in both prokaryotic and eukaryotic organisms.