What is the process of elution?

At which stage of $PCR$,does the template join with the primer?

Can you think and answer how a reporter enzyme can be used to monitor transformation of host cells by foreign $DNA$ in addition to a selectable marker?

Discuss briefly how a probe is used in molecular diagnostics.

When a foreign $DNA$ is introduced into an organism,how is it maintained in the host and how is it transferred to the progeny of the organism?

From the following tools/techniques of genetic engineering,identify those which are required for cloning a bacterial gene in animal cells and choose the correct option:
$I$. Endonuclease $II$. Ligase
$III$. $A. tumefaciens$ $IV$. Microinjection
$V$. Gene gun $VI$. Lysozyme
$VII$. Cellulase $VIII$. Electrophoresis