If the nucleotide sequence of a probe is 3'-A | vedclass

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Question

(A) probe is a single-stranded $DNA$ or $RNA$ molecule used to detect the presence of a complementary sequence in a sample.Hybridization occurs throug

Vedclass · Accepted Answer

$5'-TAGTCG-3'$

Vedclass · Answer

(A) probe is a single-stranded $DNA$ or $RNA$ molecule used to detect the presence of a complementary sequence in a sample.Hybridization occurs through base pairing between complementary nucleotides,where $A$ pairs with $T$ (or $U$ in $RNA$) and $C$ pairs with $G$.Crucially,$DNA$ strands are antiparallel,meaning the $3'-5'$ end of one strand aligns with the $5'-3'$ end of the complementary strand.Given the probe sequence: $3'-A-T-C-A-G-C-5'$.The complementary strand must be antiparallel $(5'-3')$ and follow base-pairing rules:$3' \rightarrow 5'$ (Probe)$5' \rightarrow 3'$ (Target)$A$ pairs with $T$$T$ pairs with $A$$C$ pairs with $G$$A$ pairs with $T$$G$ pairs with $C$$C$ pairs with $G$Therefore,the complementary sequence is $5'-TAGTCG-3'$.Thus,the correct option is $A$.

If the nucleotide sequence of a probe is $3'-ATCAGC-5'$,which of the following strands will it hybridize with?

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