Which of the following are considered second-generation vaccines?

  • A
    Smallpox vaccine
  • B
    $D.P.T.$ vaccine
  • C
    Hepatitis $B$ vaccine
  • D
    Polio vaccine

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Which of the following techniques serves the purpose of early diagnosis of $AIDS$,cancer,etc?
$I$. Polymerase chain reaction
$II$. Recombinant $DNA$ technology
$III$. Enzyme-linked immunosorbent assay
Choose the correct option.

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