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Restriction enzyme was discovered by

Which of the following would not be used in preparing recombinant $DNA$?

Match Column-$I$ with Column-$II$ and choose the correct combination from the options given below:
Column-$I$ Column-$II$
$(a)$ Cloning vector $(i)$ Agrobacterium
$(b)$ Ti-$DNA$ $(ii)$ pBR$322$
$(c)$ Disarmed pathogen $(iii)$ $GAATTC$
$(d)$ Recognition sequence $(iv)$ Transformation

The function of a restriction endonuclease enzyme is to:

The most thoroughly studied of the known bacteria-plant interactions is the

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