PCR is a three-step process based on temperat | vedclass

Name: Exam Paper Generator | JEE NEET Paper Generator | Vedclass
Brand: Vedclass
Rating: 5 (35 reviews)

Question

(A) $PCR$ (Polymerase Chain Reaction) involves three main steps:$1$. Denaturation: The double-stranded $DNA$ is heated to a high temperature (approx.

Vedclass · Accepted Answer

Denaturation,Annealing,Extension

Vedclass · Answer

(A) $PCR$ (Polymerase Chain Reaction) involves three main steps:$1$. Denaturation: The double-stranded $DNA$ is heated to a high temperature (approx. $94-98^{\circ}C$) to separate the strands.$2$. Annealing: The temperature is lowered (approx. $50-65^{\circ}C$) to allow primers to bind to the single-stranded $DNA$ templates.$3$. Extension: The temperature is adjusted (approx. $72^{\circ}C$) for the $Taq$ $DNA$ polymerase to synthesize the new $DNA$ strand by adding nucleotides.Therefore,the correct sequence is Denaturation,Annealing,and Extension.

$PCR$ is a three-step process based on temperature,which follows the sequence of ........

Similar Questions

If a recombinant $DNA$ molecule carrying an ampicillin resistance gene is introduced into an $E. coli$ cell and the host cells are spread on an agar plate containing ampicillin,then...

Which of the following is correct for polymerase chain reaction $(\text{PCR})$?

In which step of $PCR$ do the primers anneal to the $DNA$ template?

Transformation is a process in which ..........

$DNA$ precipitation out of a mixture of biomolecules can be achieved by treatment with

Vedclass Test Series

Exam Paper Generator

Online Exam Module