pBR-322 is frequently used as a cloning vecto | vedclass

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(B) $pBR-322$ is one of the most widely used cloning vectors in biotechnology.It is not a natural plasmid but an engineered (artificial) plasmid const

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It is an engineered bacterial plasmid.

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(B) $pBR-322$ is one of the most widely used cloning vectors in biotechnology.It is not a natural plasmid but an engineered (artificial) plasmid constructed by Bolivar and Rodriguez in $1977$ from $E. coli$ plasmids.It contains specific restriction sites,antibiotic resistance genes ($amp^R$ and $tet^R$),and an origin of replication $(ori)$,which makes it an ideal tool for gene cloning.

Column-$I$	Column-$II$
$(A) E$	$(1) 1^{st}$ in order of identification
$(B) co$	$(2)$ Name of genus
$(C) R$	$(3)$ Name of species
$(D) I$	$(4)$ Name of strain

$pBR-322$ is frequently used as a cloning vector. What is it?

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