Why is the culture continuously agitated in a rotary shaker during suspension culture?
$(i)$ To facilitate gas exchange for the cells.
$(ii)$ To ensure proper mixing of nutrients in the medium.
$(iii)$ To sterilize the equipment used in the process.
$(iv)$ To maintain a uniform temperature for the inoculum.
$(v)$ This process causes cell aggregates to break into smaller,distinct cell clusters.

  • A
    $(i), (ii)$ and $(v)$
  • B
    $(i), (ii), (iv)$
  • C
    $(i), (iii), (ii)$
  • D
    Only $(iii)$

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Assertion $A$: Growth is faster in suspension culture compared to callus culture.
Reason $R$: Agitating the culture in the medium using a rotary shaker facilitates the mixing of nutrients and gas exchange.

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