Which of the following statements is false?

  • A
    Placing the selected explant in a suitable culture medium.
  • B
    Growth promoters like $2,4-D$ are added in suspension culture.
  • C
    Gibberellins and auxins are added as growth promoters in callus culture.
  • D
    Transfer of culture to fresh glassware is called subculturing.

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Why is the culture continuously agitated in a rotary shaker during suspension culture?
$(i)$ To facilitate gas exchange for the cells.
$(ii)$ To ensure proper mixing of nutrients in the medium.
$(iii)$ To sterilize the equipment used in the process.
$(iv)$ To maintain a uniform temperature for the inoculum.
$(v)$ This process causes cell aggregates to break into smaller,distinct cell clusters.

Which growth regulators are used in the medium during callus culture?

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