Autonomously replicating circular extrachromosomal $\text{DNA}$ is called:

$A$: Disarmed $Ti$ plasmid can be utilised as a shuttle vector.
$R$: It can survive in both prokaryotic and eukaryotic organisms.

In the naming of restriction enzymes, the first letter is derived from $...A...$ name, the next two letters from the $...B...$, and the fourth letter from $...C...$ of $...D...$ where the enzymes are extracted.
$A$ to $D$ in the statement can be:

Which of the following features is $NOT$ required for a cloning vector?

Each restriction endonuclease functions by inspecting the length of a $DNA$ sequence. It cleaves $ . . . . $

$A$ plasmid