Hardening in tissue culture is

Haploid cultures can be obtained by culturing:

Why are plants obtained by protoplast culture called somatic hybrids?

The ability of any cell to generate the entire organism is known as:

Assertion $A$: In the plant tissue culture method,the nutrient content of the medium decreases.
Reason $R$: In this method,the biomass of cells or tissues increases.
Which option is correct for Assertion $A$ and Reason $R$?

Why is the culture continuously agitated in a rotary shaker during suspension culture?
$(i)$ To facilitate gas exchange for the cells.
$(ii)$ To ensure proper mixing of nutrients in the medium.
$(iii)$ To sterilize the equipment used in the process.
$(iv)$ To maintain a uniform temperature for the inoculum.
$(v)$ This process causes cell aggregates to break into smaller,distinct cell clusters.