Identify $X$ and $Y$ from the given figure of the cloning vector $pBR322$.

Recombinant $DNA$ technology is related with

Match the following:-

Column-$I$	Column-$II$
$(A)$ $\text{YAC}$	$(I)$ Separation of $\text{DNA}$ fragments
$(B)$ $\text{Hind II}$	$(II)$ Yeast
$(C)$ Gel electrophoresis	$(III)$ First restriction endonuclease
$(D)$ Heterologous host	$(IV)$ Recombinant protein

Assertion : In recombinant $DNA$ technology,human genes are often transferred into bacteria (prokaryotes) or yeast (eukaryote).
Reason : Both bacteria and yeast multiply very fast to form huge population,which express the desired gene.

Identify '$U$','$V$' and '$W$' in the given figure of $pBR322$.

Match the following techniques or instruments with their usage:

Column-$I$	Column-$II$
$(a)$ Bioreactor	$(i)$ Separation of $DNA$ fragments
$(b)$ Electrophoresis	$(ii)$ Production of large quantities of products
$(c)$ $PCR$	$(iii)$ Detection of pathogen, based on antigen-antibody reaction
$(d)$ $ELISA$	$(iv)$ Amplification of nucleic acids

Select the correct option from the following: