Match List-$I$ with List-$II$:

List-$I$	List-$II$
$A. \alpha-1$ antitrypsin	$I. \text{Cotton bollworm}$
$B. \text{Cry } IAb$	$II. ADA \text{ deficiency}$
$C. \text{Cry } IAc$	$III. \text{Emphysema}$
$D. \text{Enzyme replacement therapy}$	$IV. \text{Corn borer}$

Choose the correct answer from the options given below:

What is the demerit of using bovine insulin (from cow) and porcine insulin (from pig) in diabetic patients?

Match the following columns.

Column-$I$	Column-$II$
$(A)$ Gene therapy	$(1)$ Effort to fix a functional gene
$(B)$ Humulin	$(2)$ $A$ single-stranded $DNA$ or $RNA$ tagged with a radioactive molecule
$(C)$ Probe	$(3)$ Diagnostic test
$(D)$ $ELISA$	$(4)$ Diabetes

In $1990$,which therapy was given to a four-year-old girl suffering from adenosine deaminase $(ADA)$ deficiency?

Why are genetically engineered lymphocytes periodically introduced into the patient in the treatment of $ADA$ deficiency?

Some of the steps involved in the production of humulin are given below. Choose the correct sequence.
$(i)$ Synthesis of gene $(DNA)$ for human insulin artificially.
(ii) Culturing recombinant $E. coli$ in bioreactors.
(iii) Purification of humulin.
(iv) Insertion of human insulin gene into plasmid.
$(v)$ Introduction of recombinant plasmid into $E. coli$.
(vi) Extraction of recombinant gene product from $E. coli$.