Match the following columns:

Column $I$	Column $II$
$A$. Recombinant $DNA$	$1$. Sea weeds
$B$. Gel electrophoresis	$2$. $DNA$ staining
$C$. Ethidium bromide	$3$. Plasmid $DNA$ that has incorporated human $DNA$
$D$. Agarose	$4$. Process by which $DNA$ fragments are separated based on their size

Which of the following statements is not true?
$I.$ $DNA$, being a hydrophilic molecule, cannot pass through cell membranes.
$II.$ $Agrobacterium$ $tumefaciens$ delivers a piece of $DNA$ known as $'T-DNA'$ in the $Ti$ plasmid, which transforms normal plant cells into tumour cells to produce chemicals against pathogens.
$III.$ Retrovirus, adenovirus, and papillomavirus are also now used as cloning vectors in animals because of their ability to transform normal cells into cancerous cells.
$IV.$ In genetic engineering, $DNA$ from different sources are cut with the same restriction enzymes so that both $DNA$ fragments have the same kind of sticky ends.
Choose the correct option.

Match the following:-

Column-$I$	Column-$II$
$(A)$ $\text{YAC}$	$(I)$ Separation of $\text{DNA}$ fragments
$(B)$ $\text{Hind II}$	$(II)$ Yeast
$(C)$ Gel electrophoresis	$(III)$ First restriction endonuclease
$(D)$ Heterologous host	$(IV)$ Recombinant protein

Which of the following restriction enzymes used in recombinant $DNA$ technology produces staggered cuts (sticky ends) in $DNA$?

Which one of the following pairs is not correctly matched?

Identify the figure given below.