Which one of the following pairs is not correctly matched?

Give differences between $PCR$ and plasmid.

Assertion : In recombinant $DNA$ technology,human genes are often transferred into bacteria (prokaryotes) or yeast (eukaryote).
Reason : Both bacteria and yeast multiply very fast to form huge population,which express the desired gene.

Match List-$I$ with List-$II$.

List-$I$	List-$II$
$A$. Transformation	$I$. Restriction enzyme
$B$. Cloning site	$II$. Transfer $DNA$ to host bacteria
$C$. Selection	$III$. Replication
$D$. Ori	$IV$. Antibiotic

Choose the correct answer from the options given below:

Match the following techniques or instruments with their usage:

Column-$I$	Column-$II$
$(a)$ Bioreactor	$(i)$ Separation of $DNA$ fragments
$(b)$ Electrophoresis	$(ii)$ Production of large quantities of products
$(c)$ $PCR$	$(iii)$ Detection of pathogen, based on antigen-antibody reaction
$(d)$ $ELISA$	$(iv)$ Amplification of nucleic acids

Select the correct option from the following:

Assertion : $Agrobacterium$ $tumefaciens$ is popular in genetic engineering because this bacterium is associated with the roots of all cereal and pulse crops.
Reason : $A$ gene incorporated in the bacterial chromosomal genome gets automatically transferred to the crop with which the bacterium is associated.