Pure $DNA$ precipitated by the addition of chilled ethanol can be removed from the solution by

Which of the following range of temperature is required for denaturation,annealing of primer,and primer extension of $PCR$ respectively?

What is the first step in the Southern blot technique?

During the purification process for recombinant $DNA$ technology,the addition of chilled ethanol precipitates out:

r-$DNA$ is inserted within the coding sequence of an enzyme,$\beta$-galactosidase. This results in the inactivation of the gene for the synthesis of this enzyme,which is referred to as . . . . . . .

For selection of recombinants,insertional inactivation of antibiotic marker has been superceded by insertional inactivation of a marker gene coding for a chromogenic substrate. Give reasons.