The first human hormone prepared by using recombinant $DNA$ technology is?

Some of the steps involved in the production of humulin are given below. Choose the correct sequence.
$(i)$ Synthesis of gene $(DNA)$ for human insulin artificially.
(ii) Culturing recombinant $E. coli$ in bioreactors.
(iii) Purification of humulin.
(iv) Insertion of human insulin gene into plasmid.
$(v)$ Introduction of recombinant plasmid into $E. coli$.
(vi) Extraction of recombinant gene product from $E. coli$.

In the modern era,animal cell culture is extensively used for the production of:

Genetically engineered insulin can be obtained by

Which of the following is produced using $E. coli$?

Humulin is . . . . . . .