To isolate protoplast,one needs:

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(C) Plant cell walls are primarily composed of cellulose,hemicellulose,and pectin. To isolate a protoplast (a plant cell without a cell wall),the cell

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Both $(a)$ and $(b)$

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(C) Plant cell walls are primarily composed of cellulose,hemicellulose,and pectin. To isolate a protoplast (a plant cell without a cell wall),the cell wall must be enzymatically degraded. Cellulase is used to digest the cellulose component of the cell wall,while pectinase is used to break down the pectin component. Therefore,a combination of both pectinase and cellulase is required for the effective isolation of protoplasts.

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