Which of the following is not present in a bioreactor?

Which one of the following statements is wrong with respect to separation of $DNA$ fragments on gel electrophoresis?

Select the option that represents the correct sequence of steps in $r-DNA$ technology.
$(1)$ Insertion of $r-DNA$ into the host
$(2)$ Isolation of $DNA$
$(3)$ Ligation of $DNA$ fragment into the vector
$(4)$ Culturing the host cells in a medium at a large scale and extraction of the desired product
$(5)$ Isolation of the desired $DNA$ fragment
$(6)$ Fragmentation of $DNA$ by restriction endonucleases

Which of the following statements is incorrect?

The significance of the heat shock method in bacterial transformation is that it facilitates:

Diagrammatically represent the experimental steps in cloning and expressing a human gene (e.g.,the gene for growth hormone) in a bacterium like $E. coli$.