Which of the following may be considered as an application of biotechnology?

Match Column-$I$ with Column-$II$ and select the correct option:

Column-$I$	Column-$II$
$(a)$ $Bt$ Cotton	$(i)$ Gene therapy
$(b)$ Adenosine deaminase deficiency	$(ii)$ Cellular defense
$(c)$ $RNAi$	$(iii)$ Detecting $HIV$ infection
$(d)$ $PCR$	$(iv)$ $Bacillus$ $thuringiensis$

Which of the following is/are considered as application$(s)$ of biotechnology?
$I$. Waste treatment
$II$. Energy production
$III$. Bioremediation
$IV$. Processed food
$V$. Genetically modified crops for agriculture
$VI$. Diagnostics
$VII$. Therapeutics
Choose the correct option.

Match the following columns:

Column $I$	Column $II$
$1.$ $r-DNA$ technology	$a.$ Typhoid
$2.$ $MRI$ technique	$b.$ $AIDS$
$3.$ $ELISA$ test	$c.$ Strong magnetic field
$4.$ Widal test	$d.$ Yeast

Match the following pairs correctly and choose the right combination:

Column-$I$	Column-$II$
$A$. Escherichia coli	$1$. Nif gene
$B$. Rhizobium melilotae	$2$. Digesting hydrocarbons of crude oil
$C$. Bacillus thuringiensis	$3$. Production of human insulin
$D$. Pseudomonas putida	$4$. Biological control of fungal disease
-	$5$. Bio-insecticide

Read the following four statements $(1-4)$ and identify the two statements that contain mistakes:
$1.$ The first transgenic buffalo,Rosie,produced milk which was human alpha-lactalbumin enriched.
$2.$ Restriction enzymes are used in the isolation of $DNA$ from other macromolecules.
$3.$ Downstream processing is one of the steps of $R-DNA$ technology.
$4.$ Disarmed pathogen vectors are also used in the transfer of $R-DNA$ into the host.
Which are the two statements having mistakes?