Which one of the following vectors is used to replace the defective gene in gene therapy?

From which organism was insulin extracted in earlier times?

What is the primary objective of genetic engineering?

Which one of the following techniques does not serve the purpose of early diagnosis of a disease for its early treatment?

$MRI$ uses .......... .

Some of the steps involved in the production of humulin are given below. Choose the correct sequence.
$(i)$ Synthesis of gene $(DNA)$ for human insulin artificially.
(ii) Culturing recombinant $E. coli$ in bioreactors.
(iii) Purification of humulin.
(iv) Insertion of human insulin gene into plasmid.
$(v)$ Introduction of recombinant plasmid into $E. coli$.
(vi) Extraction of recombinant gene product from $E. coli$.