The disadvantage of using porcine insulin in diabetic patients is

Some of the steps involved in the production of humulin are given below. Choose the correct sequence.
$(i)$ Synthesis of gene $(DNA)$ for human insulin artificially.
(ii) Culturing recombinant $E. coli$ in bioreactors.
(iii) Purification of humulin.
(iv) Insertion of human insulin gene into plasmid.
$(v)$ Introduction of recombinant plasmid into $E. coli$.
(vi) Extraction of recombinant gene product from $E. coli$.

Which one of the following molecular diagnostic techniques is used to detect the presence of a pathogen in its early stage of infection?

$ELISA$ system is based on which of the following principles?

Which protein is used in the treatment of emphysema?

How many recombinant therapeutics have been approved for human use in the world over?