Identify the figure.

Assertion : In recombinant $DNA$ technology,human genes are often transferred into bacteria (prokaryotes) or yeast (eukaryote).
Reason : Both bacteria and yeast multiply very fast to form huge population,which express the desired gene.

$A$ - Transformation is a procedure through which a piece of $DNA$ is introduced in a host bacterium.
$R$ - With the help of $PCR$,thousands of $DNA$ copies are formed.

Match the columns:

Column-$I$	Column-$II$
$(a)$ Ethidium Bromide	$(i)$ Agrobacterium
$(b)$ Ti plasmid	$(ii)$ Tetracycline
$(c)$ $PvuI$	$(iii)$ Gel electrophoresis
	$(iv)$ Ampicillin

Which of the following restriction enzymes used in recombinant $DNA$ technology produces staggered cuts (sticky ends) in $DNA$?

Identify '$U$','$V$' and '$W$' in the given figure of $pBR322$.