In the presence of a chromogenic substrate,re | vedclass

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(B) In recombinant $DNA$ technology,the process of insertional inactivation is used to distinguish recombinant colonies from non-recombinant ones. The

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Colourless colonies

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(B) In recombinant $DNA$ technology,the process of insertional inactivation is used to distinguish recombinant colonies from non-recombinant ones. The enzyme $\beta$-galactosidase is encoded by the $lacZ$ gene. When a foreign $DNA$ fragment is inserted into the $lacZ$ gene,the enzyme becomes inactivated. In the presence of a chromogenic substrate,non-recombinant bacteria (containing the functional $lacZ$ gene) produce blue-coloured colonies. However,recombinant bacteria (where the $lacZ$ gene is interrupted) fail to produce the enzyme and therefore appear as colourless colonies.

In the presence of a chromogenic substrate,recombinant bacteria give:

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