Match List-$I$ with List-$II$:

List-$I$	List-$II$
$A$. Genetically modified organism	$I$. Agrobacterium tumefaciens
$B$. $DNA$ polymerase	$II$. Bt cotton
$C$. Ti plasmid	$III$. Thermus aquaticus
$D$. pBR322	$IV$. Escherichia coli

Choose the correct answer from the options given below:

Assertion : In recombinant $DNA$ technology,human genes are often transferred into bacteria (prokaryotes) or yeast (eukaryote).
Reason : Both bacteria and yeast multiply very fast to form huge population,which express the desired gene.

The production of human proteins in bacteria through genetic engineering is possible because

Match the following columns:

Column-$I$	Column-$II$
$(a)$ $Salmonella$	$(1)$ $Ti$ plasmid
$(b)$ $E. coli$	$(2)$ Thermostable $DNA$ polymerase
$(c)$ $Agrobacterium$ $tumefaciens$	$(3)$ Plasmid for first $r-DNA$
$(d)$ $Thermus$ $aquaticus$	$(4)$ $EcoR I$

Consider the following statements:
$I.$ Recombinant $DNA$ technology,popularly known as genetic engineering,is a stream of biotechnology which deals with the manipulation of genetic material by man in vitro.
$II.$ $pBR322$ is the first artificial cloning vector developed in $1977$ by Bolivar and Rodriguez from $E. coli$ plasmid.
$III.$ Restriction enzymes belong to a class of enzymes called nucleases.
Which of the statements given above are correct?

Given below are two statements: one is labelled as Assertion $(A)$ and the other is labelled as Reason $(R)$.
Assertion $(A):$
Polymerase chain reaction is used in $DNA$ amplification.
Reason $(R):$
The ampicillin resistant gene is used as a selectable marker to check transformation.
In the light of the above statements,choose the correct answer from the options given below: