Nowadays,it is possible to detect the mutated | vedclass

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(C) The technique used to detect mutated genes is based on the principle of molecular hybridization.$1$. $A$ radioactive probe is a single-stranded $D

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mutated gene does not appear on a photographic film as the probe has no complementarity with it.

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(C) The technique used to detect mutated genes is based on the principle of molecular hybridization.$1$. $A$ radioactive probe is a single-stranded $DNA$ or $RNA$ molecule tagged with a radioactive isotope.$2$. This probe is designed to be complementary to the normal gene sequence.$3$. When this probe is introduced to a clone of cells,it will hybridize (bind) to the normal gene sequence present in the cells.$4$. However,if a gene is mutated,the sequence of the mutated gene will differ from the normal gene.$5$. Due to this difference,the radioactive probe will not be able to form a stable hybrid with the mutated gene.$6$. Consequently,when the cells are exposed to a photographic film (autoradiography),the normal genes will show up as radioactive signals,while the mutated gene will not appear on the film because the probe failed to bind to it.

Nowadays,it is possible to detect the mutated gene causing cancer by allowing a radioactive probe to hybridize with its complementary $DNA$ in a clone of cells,followed by its detection using autoradiography because:

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