Restriction enzymes should not have more than one site of action in the cloning site of a vector. Comment.
(N/A) vector is designed to have a unique recognition site for a specific restriction enzyme within its cloning site. If a restriction enzyme has multiple recognition sites within the vector,the vector $DNA$ will be cleaved into multiple fragments upon treatment with that enzyme. This would disrupt the integrity of the vector,making it impossible to insert the gene of interest and successfully create a recombinant $DNA$ molecule.
Explore More
Similar Questions
$pBR322$ was the first artificial cloning vector developed in $...A...$ by $...B...$ and $...C...$ from $E. coli$ plasmid. Here $A, B$ and $C$ can be:
Easy
View SolutionThe ligation of an antibiotic resistance gene with a plasmid vector can be performed by which of the following enzymes?
Restriction endonucleases are enzymes which
Medium
View SolutionAssertion : Restriction enzymes cut the strand of $DNA$ to produce sticky ends.
Reason : Stickiness of the ends facilitates the action of the enzyme $DNA$ polymerase.
Reason : Stickiness of the ends facilitates the action of the enzyme $DNA$ polymerase.
MediumAIIMS 2009
View SolutionIdentify the labels $M$ and $N$ in the following agarose gel electrophoresis representation.
Vedclass Products
For Students
Vedclass Test Series
Mock tests in real JEE/NEET style with performance analysis. 5-day free trial.
Start Free TrialFor Teachers
Exam Paper Generator
Generate Set A/B/C/D exam papers from 7.5L+ questions in 2 minutes. 3 chapters free.
Try FreeFor Institutes
Online Exam Module
Live online exams with unlimited students, 360° analytics & white-label branding.
See Demo