While doing a PCR,the 'denaturation' step is | vedclass

Name: Exam Paper Generator | JEE NEET Paper Generator | Vedclass
Brand: Vedclass
Rating: 5 (35 reviews)

Question

(B) The $PCR$ process relies on the denaturation step to separate the double-stranded $DNA$ into single strands at high temperatures (around $94-98^{\

Vedclass · Accepted Answer

Primers will not anneal to the template $DNA$.

Vedclass · Answer

(B) The $PCR$ process relies on the denaturation step to separate the double-stranded $DNA$ into single strands at high temperatures (around $94-98^{\circ}C$).If the denaturation step is missed,the $DNA$ remains double-stranded.Consequently,the primers cannot bind (anneal) to the complementary sequences on the template $DNA$.As a result,the $DNA$ polymerase cannot initiate the extension process,and no amplification of the target $DNA$ sequence will occur.

While doing a $PCR$,the 'denaturation' step is missed. What will be its effect on the process?

Similar Questions

Pure $DNA$ precipitated by the addition of chilled ethanol can be removed from the solution by

Select the correct sequence of steps in $PCR$.

In the case of polymerase chain reaction $(PCR)$,what are the temperatures required for the following steps?
$A.$ Denaturation
$B.$ Annealing
$C.$ Extension

Identify the correct match for the given diagram.

For selection of recombinants,insertional inactivation of antibiotic marker has been superceded by insertional inactivation of a marker gene coding for a chromogenic substrate. Give reasons.

Vedclass Test Series

Exam Paper Generator

Online Exam Module